RNAscope® Pretreatment reagents are used for blocking endogenous peroxidase activity and to optimally permeabilize properly prepared samples. These reagents have been optimized to better access target RNA and reduce optimization time in multiple tissues such as formalin-fixed, paraffin-embedded (FFPE) (including archival) tissue, fresh‑frozen (FF) tissue, fixed‑frozen tissue, tissue microarray (TMA), and cell preparations. The pretreatment reagents listed in this section are for the manual RNAscope® and BaseScope™ Assays. Please refer to the appropriate sample preparation guides as shown in the User Manual Selection Guide available at here to ensure your samples are prepared correctly for the RNAscope® and BaseScope™ detection assay.

RNAscope® 2.5 Universal Pretreatment Reagents (322380): this kit includes all the pretreatment reagents that may be used across different tissue types:

- RNAscope® Target Retrieval Reagent
- RNAscope® Hydrogen Peroxide Reagent
- RNAscope® Protease III Reagent*
- RNAscope® Protease IV Reagent*
- RNAscope® Protease Plus Reagent*

All Chromogenic (Single-plex Red/Brown and Duplex) reagent kits will ship with pretreatment reagents for FFPE tissues (RNAscope® Hydrogen Peroxide, RNAscope® Target Retrieval & RNAscope® Protease Plus). The RNAscope® Fluorescent Multiplex Reagent kit will ship with pretreatment reagents for use with Fresh Frozen tissues or cultured cells (RNAscope® Protease III & RNAscope® Protease IV). The RNAscope® Multiplex Fluorescent Reagent kit v2 and BaseScope kit will ship with the Universal Pretreatment kit. Detection reagents will require ordering of pretreatment reagents listed below or the Universal Pretreatment kit.

Bio-Techne Corporation (NASDAQ: TECH) is a leading developer and manufacturer of high quality purified proteins:notably cytokines and growth factors, antibodies, immunoassays, as well as biologically active small molecule compounds and ACD's in situ hybridization detection products,which are sold to biomedical researchers and clinical research laboratories; these operations constitute the core Biotechnology Division, headquartered in Minneapolis, Minnesota. The Protein Platforms Division manufactures innovative protein analysis tools under the ProteinSimple brand name that greatly automate western blotting and immunoassay practices. The Diagnostics Division manufactures FDA-regulated controls, calibrators, blood gas and clinical chemistry controls and other reagents for OEM customer and clinical customers. Bio-Techne products are integral components of scientific investigations into biological processes and the nature and progress of specific diseases. They aid in drug discovery efforts and provide the means for accurate clinical tests and diagnoses. With thousands of products in its portfolio, Bio-Techne generated approximately $563 million in net sales in fiscal 2017 and has approximately 1,800 employees worldwide. For more information on Bio-Techne and its brands, please visit www.bio-techne.com.