Liquid Chromatography Precolumn,specification,price,image-Bio-Equip in China

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Liquid Chromatography Precolumn
COSTONES liquid chromatography pre column is a liquid chromatography column developed based on high-quality ultra pure porous spherical silica gel, which is widely used in reversed-phase and normal phase chromatographic analysis. With the rigorous production process and cutting-edge chemical bonding technology, this series of products can meet all kinds of stringent requirements from laboratory precision analysis to large-scale preparation applications.

Technical core:
 high purity matrix: ultra pure silica gel with a total content of metal ions less than 20 ppm is used to effectively eliminate the interference of metal impurities on polar compounds.
 excellent structure: it has high specific surface area and excellent mechanical strength to ensure stable column bed structure under long-term high-pressure operation.
 unique bonding technology: the unique chemical bonding and modification process gives the product excellent chemical stability and reproducibility between batches.
Core advantages
Low silicon hydroxyl activity: after deep tail sealing treatment, it can effectively inhibit the secondary retention effect and improve the peak shape of basic compounds.
Uniform ligand bonding: the surface modification is highly consistent, ensuring the stability and durability of separation selectivity.
Ultra low metal content: reduce chelation, avoid tailing, and improve the analysis accuracy of sensitive compounds.
Narrow particle size distribution: the particle size distribution range is narrow, which significantly improves the column efficiency and reduces the system back pressure.
Excellent stability: it has excellent acid and alkali resistance stability and long service life, reducing the experimental operation cost.

Reversed phase/normal phase silica gel matrix chromatographic column

Product	Particle size	Aperture	Specific surface area	Carbon content	pH
C18	3/5/10 um	100Å	300m²/g	16%	2-8
C18H	5/10 um	120Å	300m²/g	18%	2-8
C18 AQ	5/10 um	100Å	300m²/g	13%	2-8
C8	3/5/10 um	120Å	300m²/g	12%	2-8
C4-300	5/10 um	300Å	100m²/g	3%	2-8
C8-300	5/10 um	300Å	100m²/g	5%	2-8
C18-300	5/10 um	300Å	100m²/g	8%	2-8
Phenyl	3/5/10 um	120Å	300m²/g	8%	2-8
SiO2	3/5/10 um	120Å	300m²/g	-	2-8
NH2	3/5/10 um	120Å	300m²/g	4%	2-8
CN	3/5/10 um	120Å	300m²/g	7%	2-8
Diol	5/10 um	120Å	300m²/g	8%	2-8
Amide	5/10 um	120Å	300m²/g	4%	2-8

Product item No：
Chromatographic Medium 10um

10um	4.6250mm	10250mm	20250mm	30250mm	50250mm
C18	72110010004625	72110010010025	72110010020025	72110010030025	72110010050025
C18H	72210010004625	72210010010025	72210010020025	72210010030025	72210010050025
C8	72510010004625	72510010010025	72510010020025	72510010030025	72510010050025
C4-300	73010010004625	73010010010025	73010010020025	73010010030025	73010010050025
C8-300	72910010004625	72910010010025	72910010020025	72910010030025	72910010050025
C18-300	72810010004625	72810010010025	72810010020025	72810010030025	72810010050025
SiO2	72010010004625	72010010010025	72010010020025	72010010030025	72010010050025

Chromatographic Medium 5um

5um	4.6150mm	4.6250mm	10250mm	20250mm	30250mm
C18	72105010004615	72105010004625	72105010010025	72105010020025	72105010030025
C18H	72205010004615	72205010004625	72205010010025	72205010020025	72205010030025
C18 AQ	72305010004615	72305010004625	72305010010025	72305010020025	72305010030025
C8	72505010004615	72505010004625	72505010010025	72505010020025	72505010030025
C4300	73005010004615	73005010004625	73005010010025	73005010020025	73005010030025
C8Bio	72905010004615	72905010004625	72905010010025	72905010020025	72905010030025
C18Bio	72805010004615	72805010004625	72805010010025	72805010020025	72805010030025
Phenyl	70605010004615	70605010004625	70605010010025	70605010020025	70605010030025
SiO2	72005010004615	72005010004625	72005010010025	72005010020025	72005010030025
NH2	70505010004615	70505010004625	70505010010025	70505010020025	70505010030025
CN	70405010004615	70405010004625	70405010010025	70405010020025	70405010030025
Phenyl	70605010004615	70605010004625	70605010010025	70605010020025	70605010030025
Diol	70705010004615	70705010004625	70705010010025	70705010020025	70705010030025
Amide	70805010004615	70805010004625	70805010010025	70805010020025	70805010030025

Chromatographic Medium 3um

3um	2.150mm	2.1150mm	4.650mm	4.6150mm
C18	72103010002105	72103010002115	72103010004605	72103010004615
Phenyl	70603010002105	70603010002115	70603010004605	70603010004615
SiO2	72003010002105	72003010002115	72003010004605	72003010004615
NH2	70503010002105	70503010002115	70503010004605	70503010004615
CN	70403010002105	70403010002115	70403010004605	70403010004615

Application fields of preparation column

The preparation column is the core component of RP-HPLC preparation chromatography. Its core function is to enrich and purify hydrophobic/moderately hydrophobic organic compounds from gram to kilogram, and separate the crude products to high-purity monomers. It is widely used in the fields of new drug research and development, natural products, fine chemicals and so on.

1. Biopharmaceuticals

-Purification of peptide drugs: purification of GLP ‑ 1 (smegliptide, tilposide), insulin, antimicrobial peptides, growth peptides, and removal of impurities with similar structures such as missing peptides, oxidation, deamidation, etc; Large aperture C18 preparation column is specially suitable for purification of long-chain peptides, which is the mainstream scheme for industrialization of peptide bulk drugs.
-Chemical small molecule Bulk Drugs&pharmaceutical intermediates * *: purification of crude synthetic drugs, separation of related substances/impurity monomers, and acquisition of impurity reference materials; Support from laboratory semi preparation to pilot and production scale-up.
-Antibiotics and fermentation products: purification of fermentation products such as macrolides, vancomycin and bacitracin, and separation of fermentation by-products and degradation impurities.
-Oligonucleotides, protein fragments, biomarkers: preparation and separation of enzymatic protein peptides, oligosaccharides and other biomacromolecules (using 300 μ m large pore C18 packing).

2. development of natural products/traditional Chinese Medicine

-Isolation of highly active monomers from plant extracts: ginsenosides, paclitaxel, Ginkgolides, flavonoids, alkaloids, coumarins, terpenes, etc.
-Separation of traditional Chinese medicine extract and preparation of Monomer Standard for pharmacological research and new drug development. The structures of these substances are highly similar, and C18 preparation column is the preferred method to obtain high purity monomer.

3. chemistry and fine chemicals

-Purification of organic synthetic products, synthetic intermediates, unnatural amino acids, perfumes and dye monomers.
-Standard/reference preparation: preparation and enrichment of high-purity standard samples of agricultural and veterinary drugs, environmental pollutants and food additives.

4. food and cosmetics

-Purification of food functional components: anthocyanins, polyphenols, vitamin derivatives, functional oil monomers.
-Purification of cosmetic active substances: separation and purification of plant active extracts and whitening/antioxidant raw materials.

5. environmental and Life Sciences Research

-Preparation of high purity standards for environmental organic pollutants (polycyclic aromatic hydrocarbons, phenols).
-Separation and enrichment of metabolites and natural secondary metabolites at the scientific research level for mass spectrometry identification and pharmacological activity evaluation.

Welcome to inquire, pangjun@costones.com