Applications
· Ideal for harvesting fragile cells such as stem cells,neurons etc.
· Collection of intact and viable single cell suspension.
· Stem cell culture and tissue engineering culture.
· Research on extra cellular matrix.
Feature
· Made of special polymer Material·
· Temperature responsive polymer is fixed to the surface of the cultureware at nano-thickness level.
· Cells are cultured at 37°C and collectable approximately 10-30min after controlling temeperature to 20-25°C.
Description
· Collection of intact viable cells without enzymatic processing.
· Trypsin is well known for its deterioration to the functions of cells. Maintenance of their activities is possible with Cosmo’s
advanced culture ware is used for cell culture.
· Harvest cells by mere reduction of temperature.
· It completely do without trypsin when collecting cells since cell detachs by just the culture surface response.So that the
Processing would save time 80%.
· 96 multiwell format is especially suitable for cell cloning by limited dilution.

Ordering Information

Cat. No.	Name	Description
2101-035	35mm Culture Dish	20/Column
2101-135	35mm Culture Dish	100/Case
1102-096	Responses Cell Culture Plate 96well	10/pack
1102-196	Responses Cell Culture Plate 96well	50/pack

Instructions Culturing cells
· First of all, pre-warm the medium to 37°C in the incubator.
· When seeding cells, please do use the pre-warmed medium to ease attachment of cells.
· Seed cells evenly over the culture surface to prevent cells being concentrated in one area.
· The surface becomes hydrophobic above 32°C therefore cells must be cultured above this point.
· Medium change can be carried out as if using normal culture ware without polymer. Please use pre-warmed medium for change.
· When cells are difficult to attach, please use medium that contains FCS.
· Attachment time of the cells may vary depending on the type of the cell.
Detachment of cells
· The surface of the cultureware becomes hydrophilic under 32°C. Cells are easier to detach under this temperature.
· When collecting cells, lower the temperature down to approximately 20°C and wait about 30 minutes. Depending on the type of the cells cultured, they detach automatically.
· Same results can be achieved by leaving the cultureware in the refrigerator set at 4 degrees celsius for 10 minutes.
· The cells that did not come off automatically can be detached by agitating them by pipet with medium.
· Some cells may not detach completely. The results may vary by the type of the cells.
· Detachment of cells are easier when the temperature is lowered before cells become confluent.
· The product is intended for one time use only. Please do not re-use the product.
· The product is only for laboratory use.
About more advanced responses cell culture ware details , please don't hesitate to contact us:
E-mail : techservice@cosmobrand.com.cn

Cosmo was founded in 2004, an expert CDMO of sterile and non sterile for life science and medical devices. we own certified class 7 cleanroom and operated by ISO 13485 and GMP regulation.