The NucleoCounter® NC-3000™ is an advanced image cytometer utilizing fluorescence imaging to characterize cell properties. The NC-3000™ can perform high-speed cell count and viability determination and includes plug-and-play analytical assays of mammalian, yeast and insect cells. Pre-defined assays include a unique 5-minute cell cycle assay and a selection of apoptosis assays.
With the FlexiCyte™ license, the user can create protocols choosing light source, emission filters and detailed settings. The accompanying intuitive software can be used for data analysis of the results and has unlimited licenses.
The NC-3000™ benchtop image cytometer ensures flow-like analysis with high reproducibility and is calibration free.
NC-3000™ Key features:
- No rinsing or clogging
- Easy to operate – no warm-up or calibration
- CFR 21 Part 11 ready
- Automated PDF reports
- Unlimited software licenses and free software updates
- Excellent support by field application specialists
- High-speed cell counting and advanced cell analysis
- Visual inspection of cells – plot to image
- Images are saved automatically and can be re-analyzed later
- Low sample volume
- Build your own assays with up to 4 analytical channels
- Export data to FCS Express, FlowJo, etc.
Cytometry analysis in the cell lab
Visual verification of the count
When counting manually the scientist can visually verify the counting. This verification of the count can likewise be performed with the intuitive NucleoView™ software included with the NC-3000™. Scatter plots and images are interlinked making it possible to verify cell populations visually.
The accompanying NucleoView™ software allows for easy coupling between the obtained image and plots showing fluorescence for precise determination of the events to count.
Counting cells in clumps with NucleoCounter® NC-3000™
The NucleoCounter® NC-3000™ can count cells even from a sample of highly aggregated cells or cells growing in clumps. In the Aggregated Cells Assay, the total cell count derives from a lyzed aliquot of the sample (see schematic representation below). Lysis disrupts the cell membrane, dismantles clumps and releases nuclei into suspension. Nuclei are stained by DAPI and automatically counted. Because dead cells are typically less abundant and tend to detach from aggregates, the dead cell count is determined from the non-lyzed aliquot (figure below). The combination of these two measurements results in high precision and accuracy for even the most difficult clumps.
Cell cycle analysis
Cell cycle analysis is the most fundamental method to establish anomalies in cell division and thereby symptoms of cell damage. With ChemoMetec’s unique 2-step cell cycle a cell cycle can be performed in 5 minutes including treatment of cells! A cell cycle profile will readily be displayed in the accompanying PlotManager software and percentage or numbers of events in G0/G1, S-phase, G2 and sub-G1 can be identified.
Content related to the NucleoCounter® NC-3000™
Quantification of GFP transfected cells
With the NucleoCounter® NC-3000™ plug-and-play GFP transfection assay, the number of viable and dead GFP-transfected cells can be determined with high precision.
21 CFR Part 11 Ready
The NC-3000™ is ideal for regulated QC, production and even PD and R&D departments. The instrument and accompanying software can easily be integrated into existing IT networks.
User adaptable protocols with FlexiCyte™
The FlexiCyte™ module enables users to perform advanced cell analysis of a broad range of biomarkers and fluorescent proteins in mammalian cells.
Counting of Mesenchymal Stem Cells
Easy and precise determination of viability and cell count of primary mesenchymal stem cells derived from lipoaspirates and bone marrow aspirates.
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ChemoMetec is a worldwide leading manufacturer in the areas of cell counting and analysis. We are growing exponentially, striving towards our goal to be the number one provider of cell counters and analyzers in every segment of the biotech industry.
ChemoMetec specializes in developing and producing high-precision equipment that delivers consistent data for thousands of customers across biotech, pharma and academic segments.
Designed, manufactured and tested in Denmark, our instruments are built with quality materials, using expert workmanship.
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