Lipidure®-COAT Plates Effective Tool for Spheroid Culture Spheroid cell culture is typically based on the spontaneous formation of an aggregate of cells in an environment where cell-cell interactions dominate over cell-substrate interaction. This can be achieved by using low-attachment cell culture conditions.   Lipidure®-COAT plates and dishes are a top of the range solution for spheroid formation, with the Lipidure® coating providing a superior low-attachment solution for the formation of single spheroids in each well of multi-well plates. Using Lipidure-COAT dishes, it is possible to undertake large scale assays to provide sufficient materials for applications such as Western blotting or gene expression microarrays. How Does Lipidure®-COAT Work?   1. Low-adhesion surface promotes cell aggregation & spheroid formation. 2. Uses MPC Polymer: a biocompatible polymer containing Phosphorylcholine (which is found in cell membranes). 3. Completely synthetic, containing no substances of biological origin.   Cell Types Tested Spheroid formation using Lipidure® has already been demonstrated for the following cell types: human ES, mouse ES, human iPS, mouse iPS, NIH3T3, pre-adipocytes, HepG2 and other cancer cell lines.   Formation of Single, Centrally Located Spheroid on U-Bottom Plate   Superior Spheroid Formation     A single spheroid is generated in the Lipidure®-COAT U-bottom well, while numerous satellite spheroids are found in the competitor’s plates. This phenomenon shows that a lot of cells adhered to the competitor’s “low-adhesion” surface.   Spheroid Formation and Application in Anti-Cancer Drug Tests Using Lipidure®-COAT Hep G2 spheroids were formed for 5 days. Cell viability was determined by WST assay after further 2 days incubation with or without Mitomycin-C   Application Notes for Lipidure®-COAT Plates: Low Cell Binding Property Embryonic Bodies Tumorspheres Neurospheres   Product List: Cat Nr. Description Pack Size   AMS.51011615 Lipidure-Coat Dish A-90D (90mm Dish) 20 pieces AMS.51011610 Lipidure-Coat Plate A-U96 (96 well U-bottom plate) 7 pieces AMS.51011611 Lipidure-Coat Plate A-F96 (96 well Flat-bottom plate) 7 pieces AMS.51011612 Lipidure-Coat Plate A-V96 (96 well V-bottom plate) 7 pieces AMS.51011617 Lipidure-Coat Multi-Dish A-6MD (6 well plate) 7 pieces AMS.51011618 Lipidure-Coat Multi-Dish A-12MD (12 well plate) 7 pieces AMS.51011619 Lipidure-Coat Multi-Dish A-24MD (24 well plate) 7 pieces AMS.51011614 Lipidure-Coat Dish A-60D (60mm Dish) 20 pieces AMS.52000011GB1G Lipidure-CM5206 (White powder), 1g 1 g AMS.52000012GB100G Lipidure-CM5206 (White powder), 100g 100 g AMS.52000012GB10G Lipidure-CM5206 (White powder), 10g 10 g AMS.AT-384B Lipidure-Coat 384 well plate (BLACK), 1 low-adhesion plate) 1 plate AMS.51011802 Lipidure-Coat 384 well plate (CLEAR), 1 low-adhesion plate) 1 plate AMS.51011801 Lipidure-Coat 384 well plate (WHITE), 1 low-adhesion plate 1 plate Publications: Yang KC, et al. Investigating the suspension culture on aggregation and function of mouse pancreatic β-cells. J Biomed Mater Res A. 2013. 101:2273-82. Okumura N et al., β-Catenin Functions Pleiotropically in Differentiation and Tumorigenesis in Mouse Embryo-Derived Stem Cells. PLoS One. 2013. 8:e63265. Steinkamp MP et al., Ovarian Tumor Attachment, Invasion, and Vascularization Reflect Unique Microenvironments in the Peritoneum: Insights from Xenograft and Mathematical Models. Front Oncol. 2013; 3: 97. Chen YJ et al., Use of "MGE enhancers" for labeling and selection of embryonic stem cell-derived medial ganglionic eminence (MGE) progenitors and neurons. PLoS One. 2013. 8:e61956. Nasu M et al., Robust Formation and Maintenance of Continuous Stratified Cortical Neuroepithelium by Laminin-Containing Matrix in Mouse ES Cell Culture. PLoS One. 2012. 7:e53024. Koehler et al. Generation of inner ear sensory epithelia from pluripotent stem cells in 3D culture. Nature. 2013. doi: 10.1038/nature12298. [Epub ahead of print]. Eiraku et al. Self-organizing optic-cup morphogenesis in three-dimensional culture. Nature 2011. 472:51–56. Yasuda et al. Development of cystic embryoid bodies with visceral yolk-sac-like structures from mouse embryonic stem cells using low-adherence 96-well plate. J Biosci Bioeng. 2009. 107:442-6. Mogi et al. The method of mouse embryoid body establishment affects structure and developmental gene expression. Tissue Cell. 2009. 41:79-84. Eiraku et al. Self-organized formation of polarized cortical tissues from ESCs and its active manipulation by extrinsic signals. Cell Stem Cell. 2008. 3:519-32. Wataya et al., Minimization of exogenous signals in ES cell culture induces rostral hypothalamic differentiation. PNAS 2008. 105:11796-11801.

AMSBIO’s mission is to be a profitable premier provider of quality life science research reagents and services helping customers develop innovative methods, processes, products and medicines. This is achieved by offering small and medium size manufacturers, academic groups and revenue generating biotechs a unique partnership for the global market and by providing state of the art and cost effective solutions to end users and partners.

Formed in 1987 by Sandy Allan and Alex Sim the company’s first offices were in Madrid, Spain. Over the following three years further offices were opened in Italy, Switzerland and the UK. During the formative years of existence AMS helped launch the successful sales campaigns of Stratagene and Invitrogen providing them with an important foothold from which to develop their global brand expansion.