Recombinant Trypsin

Catalog Number: RPT0201

CAS: 9002-07-7
EC: 3.4.21.4

Storage temperature: 2-8℃

Source: Recombinant trypsin is a genetically engineered protein expressed in E.coli 

Protein Sequence:The amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin

 

1.DESCRIPTION

Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. YaxinBio recombinant trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin .Recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes. The M.W. of recombinant trypsin is 24KD,and the optimum pH is pH 7.0-11.0. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF,soybean trypsin inhibitor and by metal chelating agents, e.g.EDTA.

 

2.MAIN FEATURES

Source	Recombinant E. coli
Appearance	White or off white, or yellowish powder
Specific activity	≥3800 USP units/mg pro.
Purity(SDS-PAGE)	Single major band
Molecular Weight(SDS-PAGE)	24.0±2.4 kDa

UNIT DEFINITION：One USP unit of trypsin activity will produce a Delta A₂₅₃ of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

 

3.RECOMMEND USAGE

Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0. 

 

4.STABILITY OF STORAGE AND TRANSPORT

Stability of storage: Recombinant trypsin lyophilized should be stored under 2-8℃ in sealed container.

 It is stable within 24 months.After dissolved with 1mM HCl or 50mM HAC, it should be stored under -20℃. 

It is no activity loss after 10 times repeated freezing and thawing.

Stability of transport: The product is stable by blue ice insulation transport.

 

5.PRODUCT ADVANTAGE

1. Animal origin free: recombinant trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
2. Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
3. High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
4. Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
5. Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2008 quality system and GMP guidelines.
6. Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

 

6.PRODUCT USAGE

The amino acid sequence of recombinant trypsin is identical to porcine pancreas-derived trypsin,with equivalent properties compared to native trypsin .Recombinant trypsin can replace native trypsin for using in a variety of biotechnological processes,such as in insulin and vaccine production.

 

7.RELATED PRODUCTS

Recombinant carboxypeptidase B

Sequencing grade trypsin

Recombinant human trypsin

Recombinant enterokinase

 

Shanghai Yaxin Biotechnology Co. Ltd. is a biotech company founded in 2008 that focuses on the research and the production of recombinant proteins. Shanghai YaxinBio,located in Shanghai Juke Biotech Park, is ISO 9001 registered company since 2009.


Our main products are Animal Origin Free recombinant carboxypeptidase B and recombinant trypsin, which are of high importance for the production of human recombinant insulin.YaxinBio recombinant carboxypeptidase B and recombinant trypsin have no trace of other contaminating enzyme activities or enzyme inhibitors. They have been successfully used in the production process of recombinant insulin by several companies. Recombinant Trypsin can also be used in the process of cell culture.


Shanghai YaxinBio continuously strives to bring to its worldwide customers the best recombinant protein products. We will continue strengthen our product management and our internal system to meet the demands of biotech companies and scientists in the field of life sciences.