Recombinant Trypsin (USP)  

Catalog Number:RPT0202
CAS:9002-07-7
EC:3.4.21.4
Source:Porcine trypsin, expressed in E.coli
 
1.DESCRIPTION
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7.0-9.0. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF , aprotinin, soybean trypsin inhibitor and by metal chelating agents, e.g. EDTA. It is typically isolated from the pancreas of animals. Animal-derived materials are now subject to more stringent regulations, as authorities strive to ensure safer biologics and pharmaceuticals, such as in insulin and vaccine production.
Recombinant porcine trypsin is a genetically engineered protein expressed in E.coli and purified by HPLC. It is manufactured under NSF ISO 9001-2008 and in accordance to good manufacturing practice (GMP) guidelines. No animal derived products are used in the fermentation, purification, and final formulation.

2.ADVANTAGES
（1）Animal origin free:No animal-derived material is used during production.
（2）High purity:
    1) Recombinant porcine trypsin provides increased specific activity no less than 3800 USP units/mg pro.
    2) Reduce production costs while improving manufacturing progresses.

3.MAIN FEATURES 

Source	E. Coli
Appearance	Clear, Colorless to Yellowish solution
Composition	10mM HCl,20mM CaCl2, pH2.0-3.0
Protein Concentration	70 ±10 mg/ml
Specific activity	≥ 3800 USP units/mg pro.
Purity（RP-HPLC)	NLT 70% β-trysin, NMT 20% α-trypsin
Bioburden	≦100 CFU/ml

UNIT DEFINITION：One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

5.RECOMMEND USAGE 
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-9.0. 

6.STORAGE INSTRUCTIONS
Recombinant trypsin solution should be stored under -70℃. It is stable within 24 months. 

7.RELATED PRODUCTS
Recombinant trypsin (Lyophilized powder), Recombinant Human Trypsin, Sequencing Grade Modified Trypsin.  

Shanghai Yaxin Biotechnology Co. Ltd. is a biotech company founded in 2008 that focuses on the research and the production of recombinant proteins. Shanghai YaxinBio,located in Shanghai Juke Biotech Park, is ISO 9001 registered company since 2009.


Our main products are Animal Origin Free recombinant carboxypeptidase B and recombinant trypsin, which are of high importance for the production of human recombinant insulin.YaxinBio recombinant carboxypeptidase B and recombinant trypsin have no trace of other contaminating enzyme activities or enzyme inhibitors. They have been successfully used in the production process of recombinant insulin by several companies. Recombinant Trypsin can also be used in the process of cell culture.


Shanghai YaxinBio continuously strives to bring to its worldwide customers the best recombinant protein products. We will continue strengthen our product management and our internal system to meet the demands of biotech companies and scientists in the field of life sciences.