Stocked Enrichment Panels for Targeted Next Generation Sequencing
xGen® Lockdown® Panels are stocked enrichment panels for targeted next generation sequencing. Lockdown Panels consist of individually synthesized and quality controlled Lockdown Probes that have been internally validated to provide the highest level of performance.
Design Method
The design of xGen® Lockdown® Panels is typically based on 1X tiling of xGen Lockdown Probes (Figure 1).
FIGURE 1. SCHEMATIC OF 1X TILING DENSITY. Probes are tiled end-to-end to allow each base within the region of interest to be covered at least once.
Synthesis
xGen® Lockdown® Probes are synthesized individually using the IDT Ultramer® synthesis platform, which incorporates the highest coupling rate (~99.6%) in the oligonucleotide manufacturing industry to provide high quality, long oligonucleotides.
The quality of each probe is then assessed by electrospray ionization mass spectrometry (ESI-MS; Figure 2 shows a typical trace). Even probes with high GC content can be synthesized. Probes that do no pass QC are documented, unlike probes from an array pool, where there is no way of knowing beforehand of their presence or absence.
FIGURE 2. ESI-MS OF A TYPICAL XGEN® LOCKDOWN® PROBE. The trace of an xGen Lockdown Probe with 78.3% GC content is shown. Sequence: 5′ biotin–GCG GCG AGC GGA GAT CCG GGG CCT GCG CTG CGC ACT CGA GCC TGG CGG GCC GGC ACG GTG CGG GCC ATG AGC GGG GCG GTG CCC CAG GAC CTA GCG GTG AGT GGC GGC CGA GTC GGG CAC
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About IDT
Integrated DNA Technologies (IDT) is a leader in manufacturing and developing products for the research and diagnostics life science market. IDT serves the areas of academic research, biotechnology, and pharmaceutical development. IDT was founded by Dr Joseph Walder in 1987. Since then, its development has been guided by an uncompromising approach to quality, a belief in the value of good service, and a determination to minimize consumer costs.
Serving over 80,000 life sciences researchers, IDT is widely recognized as the industry leader in custom oligonucleotides due to its capabilities in:
Analytical Sophistication—IDT pioneered the use of high throughput quality control (QC) methods and is the only oligonucleotide manufacturer to offer purity guarantees and 100% QC. Every oligonucleotide is analyzed by mass spectrometry and purified oligonucleotides receive further analysis by CE and HPLC.
Design Engineering—IDT maintains an engineering division dedicated to advancing synthesis, processing technology, and automation. An in-house machine shop provides rapid prototyping and custom part design/control.
Customer Support—IDT received over 100,000 calls last year with an average wait time of only 8 seconds.
Reagent and Input Control—IDT receives all solvents in large bulk containers, runs QC on all incoming materials, and performs bulk reagent formulation and functional QC on all reagents.
IDT has over 700 employees serving our worldwide customer base from headquarters in Coralville, Iowa, and facilities in San Diego, California; Leuven, Belgium; and Singapore.
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