IDT and SGI-DNA have partnered to offer custom gene synthesis. Synthetic gene products manufactured by IDT and SGI-DNA can be ordered through the IDT website, allowing researchers to save money spent on construction and cloning, and saving significant time and effort by outsourcing the production of hard-to-clone sequences that often result in repeated failures.

All genes are constructed using the highest quality oligonucleotides from IDT. Genes arrive in a plasmid cloning vector and are ready for use in a variety of applications.

Sequence information is always secure and confidential at IDT. Non-disclosure agreements are available through IDT’s legal services upon request.

 

IDT Cloning Vectors

All synthetic gene products manufactured by IDT and SGI-DNA are delivered in a vector, ready to be transformed into E. coli.

“Best-Fit” Vectors for Genes ≤5000 bp

Occasionally, during cloning protocols, a desired DNA insert will prove to be incompatible with a predefined vector due to unforeseen cloning or toxicity issues. This can cause unpredictable delays in manufacturing and delivery of synthetic genes to researchers.

In order to optimize manufacturing and delivery time, synthetic genes ≤5 kb in length will be provided in a “best-fit” IDT cloning vector. A choice of either ampicillin or kanamycin selection markers is available.

Providing synthetic genes ≤5 kb in best-fit IDT cloning vectors is the most reliable way for IDT to provide you with your gene of interest in the shortest time. Your high-quality, synthetic gene can then be subcloned into the vector of your choice using a variety of methods. IDT cloning vectors are provided in the table below. The identity of the cloning vector used, its sequence, and insertion site will be confirmed in the documentation accompanying the product.

 

pIDTBlue Option for Genes ≤1500 bp

Genes ≤1500 bp in length may be ordered in pIDTBlue when in vitro transcription of the gene is intended. Similar to the pBluescript II phagemid vector, pIDTBlue contains a multiple cloning site in the alpha fragment of the LacZα gene and T3 and T7 promoters flanking the insertion site. PIDTBlue is Ampicillin resistant.

 

BACs and Genes >5000 bp

Genes >5000 bp are provided in a BAC with a chloramphenicol resistance marker. Instructions on how to handle and use BAC vectors can be found here.

Vector name	Size	Selection marker	Application	Sequence
pUCIDT (Amp)	2752 bp	Ampicillin	Cloning	pUCIDT (Amp).txt
pUCIDT (Kan)	3409 bp	Kanamycin	Cloning	pUCIDT (Kan).txt
pIDTSmart (Amp)	2056 bp	Ampicillin	Cloning	pIDTSmart (Amp).txt
pIDTSmart (Kan)	1962 bp	Kanamycin	Cloning	pIDTSmart (Kan).txt
pIDTBlue	2951 bp	Ampicillin	In vitro transcription	pIDTBlue.txt
pBRIDT	3170 bp	Ampicillin	Cloning	pBRIDT.txt
BAC 2	6944 bp	Chloramphenicol	Cloning	BAC 2.txt
BAC 3	8322 bp	Chloramphenicol	Cloning	BAC 3.txt

About IDT

Integrated DNA Technologies (IDT) is a leader in manufacturing and developing products for the research and diagnostics life science market. IDT serves the areas of academic research, biotechnology, and pharmaceutical development. IDT was founded by Dr Joseph Walder in 1987. Since then, its development has been guided by an uncompromising approach to quality, a belief in the value of good service, and a determination to minimize consumer costs.

Serving over 80,000 life sciences researchers, IDT is widely recognized as the industry leader in custom oligonucleotides due to its capabilities in:

Analytical Sophistication—IDT pioneered the use of high throughput quality control (QC) methods and is the only oligonucleotide manufacturer to offer purity guarantees and 100% QC. Every oligonucleotide is analyzed by mass spectrometry and purified oligonucleotides receive further analysis by CE and HPLC.
Design Engineering—IDT maintains an engineering division dedicated to advancing synthesis, processing technology, and automation. An in-house machine shop provides rapid prototyping and custom part design/control.
Customer Support—IDT received over 100,000 calls last year with an average wait time of only 8 seconds.
Reagent and Input Control—IDT receives all solvents in large bulk containers, runs QC on all incoming materials, and performs bulk reagent formulation and functional QC on all reagents.
IDT has over 700 employees serving our worldwide customer base from headquarters in Coralville, Iowa, and facilities in San Diego, California; Leuven, Belgium; and Singapore.