Intended Use
the beta-lactams & tetracyclines combo test kit is a lateral flow assay that determines a qualitative level

for the presence of both beta-lactam and tetracycline antibiotic residues in raw cow's milk. this test is

designed for rapid field use or daily control in laboratories.
Limit of detection: 

beta-lactam antibiotics
penicilins		cefalosporins
antibiotic (eu mrl)	detection limit (μg/kg)	antibiotic ( eu mrl)	detection limit (μg/kg)
penicillin g (4)	1-2	cefquinome (20)	5-7
cloxacillin (30)	3-6	cefalonium (10)	3-5
amoxicillin (4)	2-3	ceftiofur (100)	70-90
oxacillin (30)	5-7	cephapirin (60)	4-8
nafcillin (30)	7-10	cefoperazone (50)	3-5
ampicillin (4)	2-3	cefacetrile (125)	15-20
dicloxacillin (30)	3-6	cefazolin (50)	20-30
benzathine (4)	3-5
tetracycline antibiotics
antibiotic (eu mrl)	detection limit (μg/kg)	antibiotic ( eu mrl)	detection limit (μg/kg)
tetracycline (100)	7-10	doxycycline(100)	7-10
chlortetracycline(100)	7-10	oxytetracycline(100)	7-10

Assay procedure: 
the rapid strips and microwell should be at room temperature (20-25c) before usetake out the same

quantity microwells and strips as samples to be tested from the pot,and write one them an identification number.place the microwells in incubator shows 37c.pipet 200ul cold raw milk to reaction well and mix completely by pipetting up and down 5 times,and then incubate mixture for5 min.add dipsticks to reaction

well after first incubation,and then incubate the mixture for 5min again. take out the dipsticks from the microwells and remove the pad of dipstick after second incubation,then visually interpret result using the diagram or reader 
result:
negative sample (-): t line(t1) signal intensity is stronger than or the same as the signal at the c-line indicates a negative result; t line(t2) signal intensity is stronger than or the same as the signal at the c-line indicates a negative result; 
positive sample (+): signal at t line(t1) is equal or less intense compared to the c-line indicates a positive result.signal at t line(t2) is equal or less intense compared to the c line indicates a positive result.
invalid results: 
the test is invalid if no colored line appears in the c-line area even if a colored line apprears in the t-line area. re-test sample if broken lines are observed.
Storage and expiry date: 
store the kits at the temperature room 2-8c,keep them in a cool place and avoid the sunlight.please do not freeze and dampthe expiry date of kit is 12 monthspls see the expiry date and lot number from outer pack 

Meizheng Group is located in ZhongguancunScince Park of Yanqing,Beijing,Since 2007 ,The Group provides innovative solutions for food and feed safety testing,environment monitoring and related life sciences technology.
Meizheng Group offers a wide range of ELISAS,strip tests, and immunoaffinity Columns for the rapid detection of antibiotics,Mycotoxins,constituents,hormones,and other veterinary drug residues in food and feed and etc.
Meizheng Group is committed to providing customers with high-quality services,efficient and continuous improvement diagnostic products for food safety.