In laboratories and scientific research institutes, liquid nitrogen is usually used as a refrigeration medium to freeze and preserve cell samples. However, liquid nitrogen is a consumable liquid. It volatilizes when exposed to heat. Even if it is stored in a low-temperature container, it will continue to evaporate and decrease. This also shows that when freezing cell samples, when the liquid nitrogen is less than a certain range, it needs to be replenished in time.
How to refill liquid nitrogen tank? How many methods are there to replenish liquid nitrogen?
There are three methods for replenishing liquid nitrogen: manual pouring, liquid nitrogen pump replenishment, and liquid nitrogen supply tank replenishment.
1. Manual pouring of rehydration fluid
It is suitable for replenishing small-capacity tanks and can be manually lifted to replenish liquid nitrogen into the tank. However, the temperature of liquid nitrogen is -196°, which is dangerous. Once the human body comes into contact with it, it may cause frostbite.
When pouring, you can use a funnel to prevent the liquid nitrogen from being poured outside because the diameter is too small. At the same time, protective measures should be taken to avoid exposing the skin.
2. Liquid nitrogen pump rehydration
The liquid nitrogen pump uses an air pump to liquefy the liquid nitrogen in the container, forming pressure to discharge the liquid nitrogen, thereby achieving the purpose of replenishing other liquid nitrogen tanks.
In addition, there are two types of liquid nitrogen pumps, foot pumps and mechanical pumps.
The foot pump needs to use the strength of the foot to discharge the liquid nitrogen; the mechanical pump is controlled by intelligent equipment, with large liquid displacement and easy operation.
However, it should be noted that both foot pumps and mechanical pumps are only suitable for 50mm diameter liquid nitrogen tanks.
3. Replenish liquid nitrogen supply tank
Its replenishing principle is similar to that of a Dewar flask. It uses liquid nitrogen to vaporize to achieve pressurization, thereby replenishing other liquid nitrogen containers.
Compared with the Dewar flask, its working pressure is lower at 0.1Mpa, making it more worry-free and safer to operate. It is often used in laboratories to reserve liquid nitrogen.
If you have any needs, please contact TcCryo liquid nitrogen tank:
Email: tcln2container@126.com
WhatsApp: +86 18903712477
Official website:https://www.cryogenicstoragetank.com/Liquid-nitrogen-pump.html
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Henan Tianchi Biotechnology Co., Ltd. is a China-based supplier of laboratory products and technical services dedicated to the life science sector. Founded in 2016 and headquartered in Zhengzhou, China, the company serves a diverse client base across academia, research institutions, public health (CDC), livestock and agriculture, as well as the pharmaceutical industry.
We provide a comprehensive range of services, including biotech research and development, technical consulting, technology transfer, and import/export services for goods and technology. Our core product portfolio covers molecular biology reagents, immunohistochemistry (IHC) reagents, antibodies, laboratory instruments, and cryogenic liquid nitrogen tanks. This integrated offering effectively meets the diverse needs of life science laboratories for reagents, consumables, and equipment.
Furthermore, Henan Tianchi Biotechnology Co., Ltd. serves as an authorized marketing and promotion partner for TcCryo.
About TcCryo
TcCryo offers a comprehensive range of cryogenic products, including laboratory liquid nitrogen dewars, biological sample storage systems, liquid nitrogen containers for animal breeding and artificial insemination, dry shippers, liquid nitrogen supply tanks, and customized cryogenic equipment.
The brand focuses on practical product design, manufacturing quality, and application-specific solutions to meet the needs of laboratories, biobanks, livestock breeding facilities, distributors, and other B2B customers.
Manufacturing & Quality Control
The company's manufacturing capabilities cover vessel fabrication, vacuum insulation processing, and surface finishing. Its quality control processes include helium mass spectrometry leak testing, vacuum inspection, static evaporation rate testing, and final product inspection.
These manufacturing and inspection capabilities support consistent product quality and performance across its cryogenic equipment range.
Global B2B Services
TcCryo supports international customers through standard product manufacturing, OEM/ODM services, customized production, and technical product selection assistance. The company works with distributors, wholesalers, importers, and end users to provide cryogenic storage products suited to different applications and requirements.
Company Information
Company Name: Henan Tianchi Cryogenic Machinery Equipment Manufacturing Co., Ltd.
Brand: TcCryo
Brand Ownership: Registered trademark owner in China
Business Type: Manufacturer
Main Business: Liquid Nitrogen Storage Containers & Cryogenic Equipment
Manufacturing Experience: Over 20 Years
Official Website: https://www.cryogenicstoragetank.com/