Haptoglobin is a protein that in humans is encoded by the HP gene. In blood plasma, haptoglobin binds free hemoglobin (Hb) released from erythrocytes with high affinity and thereby inhibits its oxidative activity. The haptoglobin-hemoglobin complex will then be removed by the reticuloendothelial system (mostly the spleen). In clinical settings, the haptoglobin assay is used to screen for and monitor intravascular hemolytic anemia. In intravascular hemolysis free hemoglobin will be released into circulation and hence haptoglobulin will bind the Hb. This causes a decline in Hp levels. Conversely, in extravascular hemolysis the reticuloendothelial system, especially splenic monocytes, phagocytose the erythrocytes and hemoglobin is not released into circulation and hence haptoglobulin levels are normal.
Sheep Haptoglobin (HP) ELISA Kit employs a two-site sandwich ELISA to quantitate HP in samples. An antibody specific for HP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sheep Haptoglobin (HP) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/sheep-haptoglobin-hp-elisa-kit-kte110063/
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