Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]
Rat Cardiac troponin I (cTn-I) ELISA Kit employs a two-site sandwich ELISA to quantitate cTn-I in samples. An antibody specific for cTn-I has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anycTn-I present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for cTn-I is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of cTn-I bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat Cardiac troponin I (cTn-I) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/rat-cardiac-troponin-i-ctn-i-elisa-kit-kte101019/
bio-equip.cn
中文
