Carbonic anhydrase II (gene name CA2), is one of fourteen forms of humanαcarbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis.Carbonic anhydrase II is expressed at high levels in osteoclasts during bone resorption. Laitala and Vaananen (1994) demonstrated in 2 in vitro culture systems that antisense RNA and DNA blocked expression of CA II and led to decreased bone resorption. In the choroid plexus, carbonic anhydrase II supports the transport of bicarbonate ions, sodium ions, and water from the blood to the cerebrospinal fluid.
Rat Carbonic anhydrase 2 (CA2) ELISA Kit employs a two-site sandwich ELISA to quantitate CA2 in samples. An antibody specific for CA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat Carbonic anhydrase 2 (CA2) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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