Nicotinamide phosphoribosyltransferase (NAmPRTase or Nampt) also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin is an enzyme that in humans is encoded by the PBEF1 gene. This protein is the rate-limiting enzyme in the nNicotinamide adenine dinucleotide (NAD+) salvage pathway that converts nicotinamide to nicotinamide mononucleotide in mammals to enable NAD+ biosynthesis. NAMPT has also been reported to be a cytokine (PBEF) that promotes B cell maturation and inhibits neutrophil apoptosis.
Rat Nicotinamide phosphoribosyltransferase (NAMPT) ELISA Kit employs a two-site sandwich ELISA to quantitate NAMPT in samples. An antibody specific for NAMPT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNAMPT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAMPT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAMPT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Rat Nicotinamide phosphoribosyltransferase (NAMPT) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/rat-nicotinamide-phosphoribosyltransferase-nampt-elisa-kit-kte100521/
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