Mouse DNA-directed RNA polymerase I subunit RPA1 (POLR1A) ELISA Kit,Abbkine_specification/price/image

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POLR1A belongs to the RNA polymerase beta' chain family.DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition

Mouse DNA-directed RNA polymerase I subunit RPA1 (POLR1A) ELISA Kit employs a two-site sandwich ELISA to quantitate POLR1A in samples. An antibody specific for POLR1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPOLR1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POLR1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POLR1A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Mouse DNA-directed RNA polymerase I subunit RPA1 (POLR1A) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

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Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.