Nicotinamide adenine dinucleotide (NAD) is a coenzyme found in all living cells. The compound is a dinucleotide, because it consists of two nucleotides joined through their phosphate groups. One nucleotide contains an adenine base and the other nicotinamide. Nicotinamide adenine dinucleotide exists in two forms, an oxidized and reduced form abbreviated as NAD+ and NADH respectively.
Mouse Nicotinamide adenine dinucleotide (NADH) ELISA Kit employs a two-site sandwich ELISA to quantitate NADH in samples. An antibody specific for NADH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNADH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NADH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NADH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Nicotinamide adenine dinucleotide (NADH) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Find more details at http://www.abbkine.com/product/mouse-nicotinamide-adenine-dinucleotide-nadh-elisa-kit-kte70842/
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