Human Proto-oncogene serine/threonine-protein kinase pim-1 (PIM1) ELISA Kit,Abbkine_specification/price/image

Product Detail
Company Profile

Proto-oncogene serine/threonine-protein kinase Pim-1 is an enzyme encoded by the PIM1 gene. The protooncogene PIM1 encodes a protein kinase upregulated in prostate cancer (Dhanasekaran et al., 2001).Viral leukemogenesis in mice is often initiated by proviral activation of the highly conserved cellular gene Pim1. Domen et al. (1987) compared the nucleotide sequence of human and mouse PIM1 cDNAs. Meeker et al. (1987) also characterized the human PIM1 gene. The deduced amino acid sequence showed significant homology to a number of the protein kinases but did not have a transmembrane region. Studies of expression of this gene using Northern blots of human cell lines showed it to be transcribed primarily in B-lymphoid and myeloid cell lines.

Human Proto-oncogene serine/threonine-protein kinase pim-1 (PIM1) ELISA Kit employs a two-site sandwich ELISA to quantitate PIM1 in samples. An antibody specific for PIM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPIM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Proto-oncogene serine/threonine-protein kinase pim-1 (PIM1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-proto-oncogene-serine-threonine-protein-kinase-pim-1-pim1-elisa-kit-kte62646/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.