Human Peroxisomal multifunctional enzyme type 2 (HSD17B4) ELISA Kit,Abbkine_specification/price/image

Product Detail
Company Profile

The HSD17B4 gene encodes an enzyme involved in peroxisomal fatty acid beta-oxidation. It was first identified as a 17-beta-estradiol dehydrogenase. Peroxisomal beta-oxidation of fatty acids is catalyzed by 3 enzymes: acyl-CoA oxidase; the 'D-bifunctional enzyme,' with enoyl-CoA-hydratase and D-3-hydroxyacyl-CoA dehydrogenase activity, and 3-ketoacyl-CoA thiolase. See also the L-bifunctional peroxisomal protein. The D- and L-bifunctional proteins have different substrate specificities. The D-bifunctional protein catalyzes the formation of 3-ketoacyl-CoA intermediates from both straight-chain and 2-methyl-branched-chain fatty acids and also acts in shortening cholesterol for bile acid formation. In contrast, the L-specific bifunctional protein does not have the latter 2 activities.

Human Peroxisomal multifunctional enzyme type 2 (HSD17B4) ELISA Kit employs a two-site sandwich ELISA to quantitate HSD17B4 in samples. An antibody specific for HSD17B4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHSD17B4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSD17B4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSD17B4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Human Peroxisomal multifunctional enzyme type 2 (HSD17B4) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Find more details at http://www.abbkine.com/product/human-peroxisomal-multifunctional-enzyme-type-2-hsd17b4-elisa-kit-kte62386/

Abbkine Scientific Co., Ltd. was founded by a number of scientists and marketing experts in the field of life science in California in 2012. With growing demands from Asia Pacific, it move its headquarters to China. Combining cutting edge technology from United States with China's manufacturing engineering and cost advantages, we aim to provide innovative, high quality assay kits, recombinant proteins, antibodies and other research tools to accelerate life science fundamental research, drug discovery, etc.