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IDT:Dr Dan Gibson Discusses the Science of Big DNA

Clicks:385 Date:09/23/13

Read about the most recent advances in synthetic biology in the latest issue of DECODED

CORALVILLE, IA 鈥 23 September 2013 鈥 In the latest issue of DECODED, the quarterly newsletter from Integrated DNA Technologies (IDT), Dr Dan Gibson discusses the science of big DNA. Dr Gibson spent most of the last decade working at the J Craig Venter Institute, as well as Synthetic Genomics Inc. (SGI), to develop, establish, and commercialize methods to take synthetic biology beyond its current limitations. In 2010 Dr Gibson and his team produced a DNA construct of 1.1 Mb, the largest synthetic DNA sequence to date, something which had not been achievable using traditional restriction/ligation methodologies. Instead, the team developed the Gibson Assembly™ Method, which uses a unique enzyme mix to assemble DNA elements with 20鈥80 overlapping bases. Once reamplified, the population, enriched with the correct sequence, is much faster to screen. The technology makes possible the creation of 10鈥30 kb sequences鈥攖he size of an entire biological pathway鈥攊n less than a week, and without cloning into E. coli. Dr Gibson and his team are currently looking at ways in which they can refine DNA assembly methods; improve large gene delivery to host cells; and develop methods that rely less on host organisms for copying their synthetic constructs.

Read the full article 鈥楽ynthetic Life: Dr Dan Gibson Discusses the Science of Big DNA鈥 in the latest issue of DECODED. For more information please visit www.idtdna.com. Follow us on twitter @idtdna for real-time updates and insights.

 

About IDT
Integrated DNA Technologies (IDT) is the largest supplier of custom nucleic acids in the world, serving academic, government, and commercial researchers in biotechnology, clinical diagnostics, and pharmaceutical development. IDT鈥檚 primary business is the manufacture of custom, synthetic DNA and RNA oligonucleotides. Today, IDT synthesizes and ships an average of 36,000 custom oligos per day to more than 86,000 customers worldwide. IDT manufacturing locations include facilities in Coralville, Iowa; San Diego, Calif.; Leuven, Belgium; and Singapore. For more information visit www.idtdna.com.

Integrated DNA Technologies
800-328-2661 (US & Canada)
+1 319-626-8400 (outside US)
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www.idtdna.com

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