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Are SNPs Impacting Your PCR Efficiency?IDT helps users to safeguard their experiments

hits:1754     Date:07/16/13

CORALVILLE, IA 鈥 15 July 2013 鈥 Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, provides an overview in the latest issue of its quarterly newsletter, DECODED, of the importance of checking for single nucleotide polymorphisms (SNPs) when designing PCR assays. If PCR is performed using primers that overlap a SNP, the SNP can impact the progress of the PCR, and thus the accuracy of resulting data. The presence of SNPs can influence both primer and probe Tm, the efficiency of the polymerase extension, and target specificity. It is therefore essential that researchers assess whether a candidate sequence contains SNPs, and avoid designing primers and probe over SNP containing sequences. Providing researchers with peace of mind, the IDT PrimeTime® qPCR Assays are developed using a proprietary design engine that checks each sequence against up-to-date SNP and intron/exon junction locations.

To find out more about how to ensure that your PCR assays are as efficient as possible, read the article, 鈥When Designing PCR Assays, Always Check for SNPs鈥, which is available in the latest issue of DECODED. For more information please visit www.idtdna.com. Follow us on twitter @idtdna for real-time updates and insights.

About IDT

Integrated DNA Technologies (IDT) is the largest supplier of custom nucleic acids in the world, serving academic, government, and commercial researchers in biotechnology, clinical diagnostics, and pharmaceutical development. IDT鈥檚 primary business is the manufacture of custom, synthetic DNA and RNA oligonucleotides. Today, IDT synthesizes and ships an average of 36,000 custom oligos per day to more than 86,000 customers worldwide. IDT manufacturing locations include facilities in Coralville, Iowa; San Diego, Calif.; Leuven, Belgium; and Singapore. For more information visit www.idtdna.com.

Integrated DNA Technologies

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