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New Cyanine Dyes Reduce Time and Cost of Oligo Labelling

hits:811     Date:03/22/13
Link Technologies鈥 solid-supported Cyanine 540 and 650 dyes increase workflow efficiency
BELLSHILL, SCOTLAND, 21 March 2013: Link Technologies Ltd, an ISO 9001:2008 certified specialist oligonucleotide reagent manufacturer, has launched a range of high quality cyanine dye products to support the continued increase in demand for fluorescent labelling in biological imaging and molecular diagnostics. As well as introducing a new range of 3鈥-CPG solid supports modified with Cyanine 540 and Cyanine 650, Link Technologies now also manufactures and supplies the commonly used phosphoramidites, so researchers can now source an extended range of cyanine reagents produced to Link Technologies鈥 exacting standards.
Until now, 3鈥-cyanine modification of an oligonucleotide could only be done post-synthetically, normally to an amino-modified oligo, or by the use of a cyanine phosphoramidite onto a universal or modified support that will not interfere with the intended use of the oligo. With Link Technologies鈥 innovative 3鈥-modified 1000Å CPG supports, customers can add cyanine dyes directly to their oligos of interest without any additional modification step. Link Technologies鈥 cyanine dye supports therefore save time by reducing the number of experimental steps and avoid the unnecessary cost of amino-modified oligos or the inconvenience of combining cyanine phosphoramidites and universal supports.
Used as fluorescent markers in oligonucleotide synthesis, dyes are central to numerous detection techniques, such as probes for monitoring real-time PCR, fluorescence in situ hybridisation, SERRS-based DNA detection assays and FRET studies in general (e.g. DNA-protein interaction studies). 3鈥-Labelling is particularly useful in the latter where the FRET partner is incorporated either at the 5鈥-end or within the oligonucleotide sequence.
For more information on the new Cyanine 540 and Cyanine 650 phosphoramidites and supports, and other oligonucleotide reagents, please visit the Link Technologies website at www.linktech.co.uk or follow us on Twitter: @linktechdna
About Link Technologies Ltd
Link Technologies Ltd is a leading kilo-scale supplier of speciality reagents for oligonucleotide synthesis and modification, all to the ISO 9001:2008 certification. The company鈥檚 main markets are university and commercial research departments, plus, increasingly, larger Contract Manufacturing Organisations (CMOs) in the biotech sector.
Link has also forged successful links to academia working closely with many UK universities. In tandem with internal research and development, the Company has an excellent record in bringing in-licence products to market with various worldwide commercial partners.
For further information see: www.linktech.co.uk.
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